RelB contributes to the survival, migration and lymphomagenesis of B cells with constitutively active CD40 signaling.

Activation of CD40-signaling contributes to the initiation, progression and drug resistance of B cell lymphomas. We contributed to this knowledge by showing that constitutive CD40-signaling in B cells induces B cell hyperplasia and finally B cell lymphoma development in transgenic mice. CD40 activates, among others, the non-canonical NF-ĸB signaling, which is constitutively activated in several human B cell lymphomas and is therefore presumed to contribute to lymphopathogenesis. This prompted us to study the regulatory role of the non-canonical NF-ĸB transcription factor RelB in lymphomagenesis. To this end, we crossed mice expressing a constitutively active CD40 receptor in B cells with conditional RelB-KO mice. Ablation of RelB attenuated pre-malignant B cell expansion, and resulted in an impaired survival and activation of long-term CD40-stimulated B cells. Furthermore, we found that hyperactivation of non-canonical NF-кB signaling enhances the retention of B cells in the follicles of secondary lymphoid organs. RNA-Seq-analysis revealed that several genes involved in B-cell migration, survival, proliferation and cytokine signaling govern the transcriptional differences modulated by the ablation of RelB in long-term CD40-stimulated B cells. Inactivation of RelB did not abrogate lymphoma development. However, lymphomas occurred with a lower incidence and had a longer latency period. In summary, our data suggest that RelB, although it is not strictly required for malignant transformation, accelerates the lymphomagenesis of long-term CD40-stimulated B cells by regulating genes involved in migration, survival and cytokine signaling.

Spirituality, Personality, and Emotional Distress During COVID-19 Pandemic in Croatia.

This study examined the association between spiritual quality of life (QoL), spiritual coping, emotional distress, and personality during the COVID-19 pandemic lockdown in a convenience sample of Croatian adults (n = 2,860, 80.6% women). Participants completed an online questionnaire that collected information on sociodemographic characteristics, distress (the Depression, Anxiety, and Stress Scale 21), spiritual coping and spiritual QoL (the WHO Quality of Life-Spirituality, Religiousness, and Personal Beliefs), and personality (the International Personality Item Pool). The hierarchical regression analysis demonstrated that personality traits, especially emotional stability, were the most significant predictors of mental health outcomes. Spiritual coping styles were a predictor of worse, while spiritual QoL of better psychological outcomes. Results demonstrate the complex relations between different aspects of spirituality/religiosity with personality and emotional outcomes and suggest that distress motivates the engagement of spiritual coping in times of disaster.

Predictors of emotional distress during the COVID-19 pandemic; a Croatian study.

The study aimed to assess relations between coronavirus-related psychological distress and its potentially predictive factors. An online sample of 2860 Croatian adults filled in questionnaires on socio-demographic characteristics, distress (the Depression, Anxiety and Stress Scale 21), coping (the Brief COPE), personality (the International Personality Item Pool), and social support (the Duke-UNC Functional Social Support Questionnaire) during the COVID-19 lockdown and after the capital was hit by an earthquake. Results indicated that 15.9% of the respondents experienced severe to extreme depression, 10.7% severe to extreme anxiety, and 26.2% severe to extreme stress. The hierarchical regressions analysis indicated that the considered variables explained a substantial percentage of the variance in depression (51.4%), anxiety (35.2%), and stress (45.5%). Main predictors of emotional distress were lower scores of Emotional Stability, higher scores of Agreeableness, avoidant coping, lack of active coping and perceived social support. The negative effect of the earthquake was weak. Results provide information on a broad range of potentially protective or vulnerability factors that could help identify those at risk for developing coronavirus-related psychological distress. Findings suggest that promoting active coping styles and social interactions could be preventive and potentially therapeutic in general populations.